1921
Volume 35, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Abstract

Studies were designed to examine skin test responses to leishmanial antigens in American visceral leishmaniasis (AVL) in Brazil. We found that after recovery from AVL, patients had positive delayed hypersensitivity reactions to . Different amounts of a soluble extract obtained from promastigotes were compared with whole promastigotes in persons with past AVL. The most effective soluble preparations tested contained 25 and 50 µg leishmanial protein. These produced positive responses in 95%–100% of the individuals with past AVL. The 25 µg protein dose was used in further studies. This preparation produced no positive responses in either normal controls, tuberculosis patients, or schistosomiasis patients, and <5% positive responses in persons with Chagas' disease. The same amount of soluble extract prepared from produced 82% positive skin test responses in persons with past AVL. When persons living in an area endemic for AVL were skin tested with the 25 µg preparation of extract, 34.1% yielded positive tests with a low number of positive responses in young children and 48% positive in adults. Only 3.1% of the population studied had a history of AVL. We have found that positive delayed hypersensitivity response to a soluble extract is a sensitive and specific indicator of previous infection with AVL.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1986.35.79
1986-01-01
2017-11-18
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1986.35.79
Loading
  • Accepted : 09 Aug 1985

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error