1921
Volume 35, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Abstract

An enzyme-inked immunosorbent assay (ELISA) using the phenolic glycolipid-1 (PGL-1) antigen of and cross-reactive antisera specific for human IgM was developed to detect IgM antibodies to in the nine-banded armadillo (). Statistical definitions for positive and negative interpretations in the ELISA were developed by screening animals recently captured and experimentally inoculated with . The ELISA was shown to have high sensitivity and specificity. Modern day armadillos of central Louisiana were observed to have a PGL-1 antibody prevalence rate as high as 20%, and a clinical disease rate as high as 5%. A retrospective serological survey of 182 armadillos taken in the years 1960–1964 and predating the use of armadillos in leprosy research was used to evaluate the 1968 environmental contamination hypothesis for the origin of infections in the wild armadillo. Antibodies to the apparently species-specific PGL-1 antigen were detected in 17 of the samples taken in 1960–1964. Absorption with whole , BCG, and 2 new armadillo-derived mycobacterial species showed these antibody reactions to be specific for PGL-1. Apparently, was enzootic in armadillos as early as 1961, and original infection of these animals could not have occurred in 1968.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1986.35.588
1986-05-01
2017-09-23
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1986.35.588
Loading
  • Accepted : 11 Jan 1986

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error