Volume 35, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Introduction of the fluorescent group lissamine rhodamine B into the surface of schistosomula of was achieved by brief incubation of the worms with liposomes carrying the lipid bound fluorophore in their bilayers. The liposomers were made of egg lecithin (PC) and lissamine-phosphatidylethanolamine (lissamine-PE). The lissamine groups could be directly detected on the parasite membrane by observation of the single worms under a fluorescent microscope. The fluorescent marker was employed to measure the lateral diffusion coefficient (D) of lipids in the schistosomular membrane and as a surface marker during the isolation of the schistosomula surface membrane.


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