1921
Volume 35, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

We describe a rapid, efficient method for purifying microfilariae from blood that does not affect their longterm motility. It is based on the separation of microfilariae on a preformed discontinuous Percoll gradient, followed by filtration on cellulose ester membranes. The parasite population, after isolation, is close to 100% pure, 100% viable and remains motile for at least 20 days. Recovery from original blood samples is typically around 90%. The method, because of its simplicity and minimal material requirements, could be used with up to 210 ml of blood. It also allows the isolation of microfilariae.

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/content/journals/10.4269/ajtmh.1986.35.148
1986-01-01
2017-11-22
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1986.35.148
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  • Accepted : 29 Jul 1985

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