Volume 35, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Studies done comparing the reactivity of serum from an infected rabbit at different intervals of infection with a crude tegument extract, excretory-secretory (ES) products and Fh are 2 by ELISA show that the first 2 antigen preparations offer a better sensitivity for detecting acute infection, especially in its early stages (3 weeks). The ES proteins from adult worms were identified by the enzyme-linked immunotransfer blot (EITB) in order to define the antigens recognized by the sera from rabbits with experimental fascioliasis. A group of 7 polypeptides with molecular weights of 23–28 Kd were the major antigens recognized. Reactivity to these antigens is maximum at 8–10 weeks of infection by EITB. As the infection progresses, reactivity to the 23–28 Kd antigens decreases but does persist through 52 weeks of infection. At least 5 other polypeptides of 120, 84, 58, 52, 39 and 33 Kd were recognized by the sera of infected rabbits from 6 to 52 weeks of infection. A different pattern was observed in parallel studies done with these same sera by immunoprecipitation of ES antigens. In addition to a 33 Kd antigen, which was detected by both techniques, a 62 Kd antigen was detected early in infection (5 weeks) and 3 major antigens of 38, 40 and 44 Kd were prominent by immunoprecipitation from 9 weeks onward. This implies the presence of multiple antigens with serodiagnostic potential. The expression of these antigens during development and their possible role in immunity is discussed.


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