1921
Volume 35, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

The ability of serum samples obtained from humans with schistosomiasis mansoni to recognize soluble egg antigens (SmSEA) was assessed by the enzyme-linked immunotransfer blot (EITB) method. The sera from 15 infected patients before and 2 years after treatment with oxamniquine (n = 30) recognized bands ranging in molecular weight from 9 to 200 Kd. A 31 Kd component of SmSEA was recognized by all infection sera, but not by normal human serum. The sera from 2 humans infected with and 2 with also recognized the 31 Kd band present in SmSEA, whereas those from 2 humans infected with did not. The 31 Kd antigen was isolated by electrophoresing SmSEA through a 15% SDS-acrylamide gel, followed by excision and electroelution of the 31 Kd band. The purified 31 Kd was used in conjunction with ELISA at a concentration of 1 µg/ml to confirm the apparent genus specificity of this protein. Thus, the sera of patients infected with , or were clearly reactive in ELISA, whereas normal human serum or sera from patients with were negative. In conclusion, the 31 Kd protein appears to be a good candidate for developing a screening assay for the immunodiagnosis of schistosomiasis.

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/content/journals/10.4269/ajtmh.1986.35.1198
1986-11-01
2017-11-23
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1986.35.1198
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  • Accepted : 09 May 1986

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