1921
Volume 34, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

A labeled synthetic polynucleotide representing a repetitive sequence from was hybridized with genomic DNA spotted on nitrocellulose. After an overnight exposure, 0.1 ng of DNA was specifically detected and 0.01 ng was detected after an exposure of 1 week. The synthetic probe showed no cross-hybridization with host DNA or with DNA isolated from other species in the phylum Apicomplexa, and species. Since synthetic DNA is easily prepared, the observed sensitivity and specificity suggests that synthetic DNA probes would be generally useful in diagnosis.

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/content/journals/10.4269/ajtmh.1985.34.837
1985-09-01
2017-11-20
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1985.34.837
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  • Accepted : 20 Mar 1985

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