1921
Volume 34, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Abstract

An enzyme-linked immunosorbent assay (ELISA) was used to measure the quantity of Rift Valley fever (RVF) viral antigen in infected Egyptian . Infectivity, as measured by plaque assay, was significantly correlated with viral antigen, as measured by the ELISA, in all groups of mosquitoes regardless of the time interval after the infectious blood meal. The proportion of noninfectious viral antigen in these groups increased with time. When individual mosquitoes were assayed the plaque assay and the ELISA techniques had similar sensitivity (100% vs. 93%, respectively) and specificity (94% vs. 94%, respectively) in detecting mosquitoes capable of transmitting virus to susceptible hamsters. The ELISA may be useful in detecting RVF-infected arthropods in the field because it provides a rapid, sensitive, and specific test.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1985.34.400
1985-03-01
2017-11-24
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1985.34.400
Loading
  • Accepted : 26 Sep 1984

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error