1921
Volume 34, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

We report the characterization of (, and ) kinetoplast DNA (kDNA) by the use of restriction endonuclease digestion patterns and Southern hybridizations. Overall, the sizes and fragment patterns of MspI restriction endonuclease-produced DNA fragments vary from species to species. However, kDNA isolates from different species and strains cross-reacted to a great extent in Southern hybridization experiments. Only kDNA isolated from and had little homology during hybridization reactions. To prepare DNA probes that would differentiate between species of , minicircle kDNA was digested with restriction enzymes and ligated to an plasmid. Several plasmids were isolated that specifically detect in hybridization experiments as few as 5 × 10 or promastigotes lysed on nitrocellulose filters.

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/content/journals/10.4269/ajtmh.1985.34.257
1985-03-01
2017-11-24
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1985.34.257
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  • Accepted : 11 Aug 1984

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