Volume 33, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Cell surface carbohydrates of , and were analyzed by a micro-agglutination assay employing 27 highly purified lectins and by binding assays using various I-labeled lectins. The following seven lectins discriminated between the trypanosomes: 1) tomato lectin (an N-acetyl--glucosamine-binding protein), both in purified form and as crude tomato juice; 2) and lectins (both N-acetyl--galactosamine-binding proteins), which selectively agglutinated ; 3) (an N-acetyl--galactosamine-binding protein) which was specific for ; 4) peanut lectin (a D-galactose-binding protein) both in purified form and as crude saline extract; and 5) and (both -fucose-binding proteins) lectins which reacted only with . Binding studies with I-labeled lectins were performed to find whether unagglutinated cells of the three different species of trypanosomes might have receptors for these lectins, in which case absence of agglutination could be due to a peculiar arrangement of the receptors. These assays essentially confirmed the agglutination experiments.


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