1921
Volume 33, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

Three murine monoclonal antibodies (I-35/67, II-190/30, III-160/18) produced by immunization against total epimastigote extract or component 5-enriched fractions of (Tehuantepec strain) have been demonstrated to recognize the component 5 specific for . By immunofluorescence studies, one of these monoclonal antibodies (I-35/67) was shown to bind to the epimastigote cell surface, and the two others (II-190/30 and III-160/18) were preferentially directed against internal subcellular organelles. Immunoprecipitation using these monoclonal antibodies followed by SDS-PAGE analysis, has led to identification of four molecules with apparent molecular weights of 72 Kd, 51 Kd, 43 Kd, and 24 Kd in epimastigotes. Potential uses of these monoclonal antibodies in serological tests and immunochemical analysis of target antigens are discussed.

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/content/journals/10.4269/ajtmh.1984.33.560
1984-07-01
2017-09-26
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1984.33.560
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  • Accepted : 22 Dec 1983

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