Volume 33, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A radioimmunoassay (RIA) originally designed to measure antibody responses to in mice was adapted for use in the immunodiagnosis of Chagas' disease in humans. The assay utilizes biotinylated antibodies and H-avidin as the tracer molecules, and has proven to be both safe and sensitive. Results using the RIA and those from direct agglutination and indirect fluorescent antibody tests were comparable in most cases. Using the RIA, we were able to discriminate between mice infected with and . Also, sera from -infected individuals do not have detectable levels of antibodies capable of binding to . Intact, fixed epimastigotes of are used as the detecting antigen in the RIA and give results comparable to those obtained with intact trypomastigotes.


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  • Accepted : 01 Jun 1983

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