1921
Volume 33, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Abstract

Cryoultramicrotomy, an antisporozoite monoclonal antibody (2G3) and protein A-gold particles were used to determine the distribution of the protective surface antigen (Pk 42) of sporozoites of and its precursors (Pk 52 and Pk 50). Gold particles bound uniformly to both the outer plasma and the inner pellicular membranes, indicating a uniform distribution of antigen on these membranes. Intracellular gold particles were frequently found to be associated with micronemes, but less frequently with rhoptries. Limited proteolysis of sporozoites totally eliminated binding of label to the outer plasma membrane. In contrast, distribution of label on the inner pellicular membranes, rhoptries, and micronemes of trypsinized sporozoites was relatively unaltered. On the basis of the present and earlier data obtained upon metabolic labeling of these parasites, it is likely that the antigen associated with the intracellular organelles corresponds to the precursor polypeptides Pk 52 and Pk 50.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1984.33.220
1984-03-01
2017-11-23
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1984.33.220
Loading
  • Accepted : 22 Sep 1983

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error