Volume 33, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The isolation of dengue 4 virus from adult , reared from eggs collected in nature, is reported for the first time. From the locality where the isolate was made, 25 pools consisting of 1,848 reared from eggs were processed. In this study, 10 different localities were sampled and a total of 10,957 adults, collected as eggs or larvae in nature, were processed for virus isolation. From a total of 158 mosquito pools tested, one recovery of dengue 4 virus was made. The isolation of dengue 4 virus from this field-collected material gives further evidence that transovarial transmission of dengue viruses occurs in nature.


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