1921
Volume 33, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

Pentamidine is an antileishmanial agent that is often toxic at therapeutic dosages. The obligate intramacrophage localization of indicates that encapsulation of pentamidine within a carrier phagocytized by macrophages (IgG-coated sheep red cell ghosts) might improve activity. In in vitro experiments, treatment of infected mouse macrophages for 1 hour with a mean of 1.4 µg of encapsulated drug resulted in a calculated drug concentration of 180 µg/ml macrophage, and in 73% suppression of organism multiplication within the macrophages after 4–5 days of further cultivation. In comparison, 27 µg unencapsulated drug/ml was needed for similar suppression. Electron microscopic examination 5 hours after phagocystosis of IgG-ghosts revealed that 95% of organisms were adjacent to ghosts in phagolysosomes. Fusion of drug carrier with phagolysosome containing drug target is therefore an important step in carrier-mediated parasite suppression in this model. These results suggest that IgG-coated erythrocyte ghosts containing pentamidine have potential as an antileishmanial formation.

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/content/journals/10.4269/ajtmh.1984.33.1112
1984-11-01
2017-09-20
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1984.33.1112
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  • Accepted : 15 May 1984

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