1921
Volume 32, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Abstract

Methods are described for the cryopreservation of third-stage larvae of . Optimum conditions utilized larvae free from the mosquito host frozen at the rate of -1° or -0.8°C per min in medium containing 9% dimethyl sulfoxide and 0.004 M polyvinylpyrrolidone. Nonfrozen or thawed larvae were inoculated intraperitoneally into jirds (), the thawed larvae after cryogenic storage for 5–378 days. In general, the percentage of adult worms recovered at necropsy were comparable between the two groups and ranged from a mean of 6–9% of the larval inoculum. In addition, three of four patas monkeys () inoculated with thawed larvae developed patent infections. The cryopreservation of third-stage larvae of also is discussed.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1983.32.767
1983-07-01
2017-11-18
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1983.32.767
Loading
  • Accepted : 09 Dec 1982

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error