Volume 32, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Rabbits were immunized with exoantigens from the spent medium of cultures, and the resultant immunologic responses were studied by indirect fluorescent antibody (IFA), hemagglutination (HA), and two-dimensional crossed immunoelectrophoretic (IEP) techniques. By crossed IEP, three parasite antigens, identified and characterized as proteins lacking lipid and carbohydrate moieties, reacted with rabbit antiserum and human immune serum. Pre-immunization sera of the rabbits used in these experiments had 1:80 IFA titers against schizonts which were then boosted eightfold by immunization with parasite exoantigens, in contrast to IFA titers of <1:2 for coccidia- and -free rabbits. Experimental infections of coccidia- and -free rabbits with rabbit spp. resulted in 1:80 anti- IFA titers, suggesting cross-reactivity of coccidial and plasmodial antigens. Post-immunization sera demonstrated extremely high HA titers against human erythrocytes, underscoring the potency of human blood components containing parasite antigens prepared from cultures. These results suggest that coccidia- and -free rabbits may be useful in the analysis of antigen of cultured .


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