1921
Volume 32, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

Synchronous growth of axenic was induced by nutritional deprivation and/or hydroxyurea. Axenic amebae maintained in 2% serum culture medium for 17–25 hours and returned to complete TP-S-1 (10% serum) medium showed a single synchronous cycle with a mean division time of 8.8 hours and a synchrony index of 0.47. Exposure to 10 mM hydroxyurea for 20–26 hours induced a single synchronous cycle with a division time of 3.0 hours and a synchrony index of 0.85. Hydroxyurea was toxic to 56% of the cells in the culture. The control generation time in TP-S-1 medium was 14.8 hours. Cells maintained for 44 hours in a nucleotide-deficient medium followed by a 10 mM hydroxyurea treatment exhibited two synchronous cycles. The first cycle had a generation time of 5.0 hours, a division time of 2.2 hours, and a synchrony index of 0.84.

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/content/journals/10.4269/ajtmh.1983.32.507
1983-05-01
2017-09-24
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1983.32.507
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  • Accepted : 20 Aug 1982

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