1921
Volume 32, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

The presence of cross-reacting antigens between , and adult worms was demonstrated by Ouchterlony immunodiffusion and enzyme-linked immunosorbent assay (ELISA). A serum bank was developed against the three trematode genera to serve as probes to determine the presence of cross-reacting antibodies to worm extracts. In this manner, it was possible to demonstrate that antigens common to and tegument were also present in worm extracts. Likewise, it was possible to demonstrate that the antigens which bind to Concanavalin A, as well as the subfraction which in isoelectric focusing has a pI of 4.2, were also found in worms. Also, a monospecific polyclonal serum to a cross-reacting antigen and the anti- serum both reacted in Ouchterlony immunodiffusion with the antigenic extract, each producing a line which linked with each other indicating common antigenic determinants and suggesting a common antigen among the digenetic trematodes. Finally, the antigenic extracts induced in mice the production of antibodies which reacted with adult worm antigens by ELISA. As all of the and sera were prepared against antigenic preparations which induced in mice protection to challenge infection with , this suggested that the worms also contain protective antigens against . Immunity to infection was induced in mice by vaccination with whole worm extracts (PwWWE). Immunized mice showed as high as a 67% worm burden reduction over controls. High doses of PwWWE did not confer protection to infection. Thus, in this study, immunity in heterologous systems was demonstrated and the existence of a common protective antigen shared by the digenetic trematodes was suggested.

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/content/journals/10.4269/ajtmh.1983.32.350
1983-03-01
2017-11-24
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1983.32.350
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  • Accepted : 30 Jun 1982

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