Volume 32, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Of three species of Nearctic black flies tested, Hagen proved the most efficient laboratory vector of the bovine parasite . Among flies inoculated intrathoracically with 40 microfilariae, numbers of 3rd-stage larvae per fly were 7.63 for , 7.54 for Zetterstedt, and 0.83 for Walker. survived the longest under laboratory conditions, with 83.3% of the females remaining alive 10 days after inoculation with 40 microfilariae of . Using an artificial membrane feeding system, could be routinely infected with by mouth. This black fly was also susceptible to infection with the Guatemalan strain of . Among flies injected with 10 microfilariae the rate of infection with 3rd-stage larvae was 93%, with a mean of three 3rd-stage larvae per fly. Successful techniques for the large-scale recovery and cryopreservation of 3rd-stage larvae of were also developed. A motility rate of 92.7% was observed in larvae cryopreserved within vector black flies.


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