1921
Volume 32, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

A radioimmunoassay (RIA) has been developed for the detection of in infected blood. The assay is based on the ability of solubilized, infected red blood cells (RBC) ( “antigen”) to combine with anti- antibodies and thus prevent the subsequent interaction of the latter with “antigen”-coated microtiter plates. A preliminary trial was carried out in Thailand to determine the usefulness of the RIA for the immunodiagnosis of malaria. Blood samples from malarious and non-malarious patients were examined both by standard microscopy and by RIA. Efficient solubilization of the parasites proved to be a major requirement for the successful performance of the RIA. Sonication or freezing and thawing, which were perfectly satisfactory for the solubilization of cultured, infected RBC, were found to be totally inadequate when applied to RBC taken from patients. However, parasites in RBC from patients could be solubilized efficiently by treatment with detergents (e.g., NP40, Triton X-100, etc.). Of the 108 blood samples tested, 23 were found positive for falciparum parasitemia by microscopy and 39 by RIA. One sample from a patient with patent falciparum parasitemia and three with patent vivax parasitemia were negative by RIA. Ten of the samples positive only by RIA belonged to patients with recent malarial infection, as shown by microscopy. Thus, the RIA detected almost all of the patients with microscopic evidence of falciparum malaria. The proportion of false positives in the RIA test was low.

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/content/journals/10.4269/ajtmh.1983.32.11
1983-01-01
2017-09-25
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1983.32.11
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  • Accepted : 13 May 1982

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