1921
Volume 30, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Abstract

Several factors that were suspected of affecting the development of syncytia in cultured cells inoculated with dengue viruses were studied. The results indicated that fresh media (less than 1 week old), low cell density at inoculation (2.8 × 10 cells/cm), and low cell passage level (less than 52 passages) favored the development of syncytia. All three types (1, 2, and 3) of dengue viruses tested could be isolated from human sera by cell culture by using syncytial development as an indicator, but the isolation rate was lower than that obtained by using intrathoracically inoculated mosquitoes.

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/content/journals/10.4269/ajtmh.1981.30.870
1981-07-01
2017-11-21
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http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1981.30.870
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  • Accepted : 10 Jan 1981

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