Volume 29, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Dengue virus isolates have been grown in the Igarashi clone C6/36 cells in amounts suitable for biochemical studies. Oligonucleotide fingerprint analyses have been used to characterize the virion 40 RNA species of the four prototype dengue (DEN) serotype viruses: DEN 1, Hawaiian strain; DEN 2, New Guinea C strain (both insect and mouse brain passaged derivatives); DEN 3, H-87 strain; and DEN 4, H-241 strain. Comparisons of the largest oligonucleotides derived by ribonuclease T1 digestion of these 40 DEN virion RNA species indicate that there are few, if any, large oligonucleotides that are homologous between any two of the four dengue prototype strains. Preliminary analyses of the 40 RNA 5′ terminal sequence of DEN 3 virion RNA indicate that it has the composition of m7GpppAmpXp.


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