Volume 29, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


In writing this manual the authors' stated objective was to present diagnostic parasitology in a clear, concise manner, for use as a bench manual to be supplemented by other sources. Garcia and Ash have succeeded in meeting their objective. This second edition is a thorough revision and expansion of the first edition. The manual is arranged in 12 chapters, an appendix, glossary and references, general and supplemental.

Chapters 1–3 present standard methodologies for collection, preservation, macro- and microscopic examination of fecal specimens and permanent staining of smears. Advantages and disadvantages of methods cited are discussed and the reader is informed why it is necessary to do specific procedures. References are cited for each technique as an aid if problems are encountered or detailed information is desired.

Chapters 4–6 detail methodologies for culture, concentration and recovery of larval nematodes, estimation of worm burden, methodology for collection and examination of specimens from the digestive and urogenital systems, sputum aspirates and biopsy material.


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