Volume 29, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A micro enzyme-linked immunosorbent assay (ELISA) for antibodies to was evaluated and the results obtained by ELISA were compared with those obtained by the complement fixation test (CF) and indirect fluorescent antibody test (IFA). Fifty sera collected from residents of the southeastern United States all had reciprocal ELISA titers ⩽320. Similarly, serum samples from 17 patients with infection proven by xenodiagnosis had reciprocal ELISA titers of ⩾1,280. Specimens from 302 El Salvador Army recruits were tested by ELISA, IFA, and CF. Excellent correlation was observed between results obtained by the three serologic tests; 62.9% of the samples were negative by each of the three tests and 24.5% were positive by all. Overall, 29.5% of the sera were positive for antibodies to by ELISA, 29.5% by IFA, and 31.5% by CF. The data suggest that the micro ELISA is a promising serologic test for measuring antibodies to in individuals and in populations.


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