Volume 28, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Preparations of eggshells of and were hydrolyzed and analyzed for amino acid composition. Both species showed great similarities in the proportions of each residue found. The predominant amino acid in shell hydrolysates was found to be glycine, which accounted for 37% of and 45% of amino acids. Four components (glycine, aspartic acid, lysine, and serine) totalled 68–75% of amino acids in the eggshells. Other individual amino acids were present in relatively small proportions ranging from 5.2–0.01%. Less than 1% of the amino acid residues were identified as tyrosine, and bityrosine was detected at a level not exceeding 1 in 1,600 residues. Carbohydrates were estimated to comprise 7.5–10% of the eggshell weight, based on hexose assay, and glucosamine was identified as the principal amino sugar in shell hydrolysates. In vivo labelling of the eggshell was demonstrated following injection of C-glycine and C-tyrosine into infected mice and subsequent purification of the shells of eggs recovered from their liver.


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