1921
Volume 27, Issue 2_Part_2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

D. K. Lvov, Institute of Virology, USSR Acad. of Med. Sciences, Gamaleya, 16, Moscow, USSR, 30 July 1975.

Ungrouped.

Isolated by D. K. Lvov et al. at Moscow, USSR, from pool of 10 adult ticks collected by hand from nest substrates on 2 September 1972 at Paramushir island, Sea of Okhotsk, USSR, Lat. 5l°16'N, Long. 155°20'E. Area of seabird colonies. Stored alive in refrigerator at 4°C.

Ticks ground in Hanks' balanced salt solution containing 10% rabbit serum. Inoculated 0.01 ml ic into 2-day-old mice.

Virus reisolated. Also virus isolate different from those existing in the laboratory.

Filterable through Milhipore 50 nm but not 100 nm. RNA as determined by lack of sensitivity of virus to 5-bromo-2-deoxyuridine. Sensitive to 50% ether; control titer 10, after-treatment titer <10. Sensitive to 1:1,000 sodium deoxycholate; controltiter 10, after-treatment titer <10. No hemagglutinin detected employing infected suckling mouse brain extracted with sucrose acetone and tested with goose erythrocytes at pH 5.5 through 7.0 and at temperatures of 4°C and 22°C.

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/content/journals/10.4269/ajtmh.1978.27.411
1978-03-01
2017-11-23
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