Volume 27, Issue 2_Part_2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


J. P. Digoutte, Institut Pasteur, B.P. 304, Cayenne, French Guiana, 30 May 1975.


Isolated by J. P. Digoutte and M. Germain at Institut Pasteur, Bangui, from pool of female mosquitoes collected on 26 April 1972 by use of human bait at Bangui, Central African Republic, Let. 4°22'N, Long. 18°35'E. Park of the Institut Pasteur in the town of Bangui. Stored in electrical deep freezer at -75°C.

Mosquitoes ground in Earle's balanced salt solution containing bovine albumin and antibiotics.

Reisolation not tried but first virus of this type in the laboratory.

Virus filterable through Millipore 220 nm. No data. Sensitive to chloroform; control titer 10 after-treatment titer <10. Hemagglutinin detected in test with infected suckling mouse brains extracted with sucrose-acetone and tested with goose erythrocytes at pH 5.7 through 6.6 and at room temperature. Optimal pH was 6.2.


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