1921
Volume 27, Issue 2_Part_2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

N. F. Stanley, Department of Microbiology, University of Western Australia, Perth Medical Centre, Shenton Park, Western Australia, 6008,2 March 1977.

Ungrouped.

Isolated by N. F. Stanley at Perth, Western Australia, from a pool of 39 adult female mosquitoes, collected with chicken-baited traps in April-May 1973 at Kununurra, Western Australia, Lat. 128°45'S, Long. 15°47'E. Open forest-grassland near bank of lagoon 1 km from town-site. Liquid nitrogen and electrical deep freezer at -70°C.

Mosquito pool ground in PBS with 0.75% bovine albumin. Suspension inoculated ic into 1-day-old infant mice on 8 November 1973.

Virus not reisolated, but first virus of this type in the laboratory.

70 × 130 nm bullet-shapedvirion by electronmicroscopy. RNA on the basis of acridine orange staining. Sensitive to 1:1 ether; control titer 10, after-treatment titer <1010. Sensitive to 1:1,000 sodium deoxycho late; control titer 10 after-treatment titer <101.0. No hemagglutinin detected using infected infant mouse brain extracted with sucrose-acetone and tested with gander erythrocytes at pH 5.9 through 7.4, at 37°C.

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/content/journals/10.4269/ajtmh.1978.27.400
1978-03-01
2017-09-21
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