Volume 26, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A complement-fixing (CF) antigen was prepared from amastigotes and trypomastigotes of (Ernestina strain) grown in beef embryo cell cultures. Multiple lots of the antigen, which consisted of a supernate of washed and disrupted organisms, required material from 10 to 10 total organisms per ml for optimum CF activity. Antibody at dilutions up to 1:256 was demonstrable in various sera from infected animals or patients. Contaminating beef cells from infected cultures were shown to be partly responsible for cross-reactions of the antigen by CF with sera from cases of cutaneous leishmaniasis in whom concomitant infection with could be excluded. There were no cross-reactions with syphilitic sera and the frequency of positive reactions with normal sera was very low. Some characteristics of the antigen included stability to storage at -20° C and -70° C for months, inactivation at 60° C and by lyophilization, and an estimated molecular size of between 50,000 and 100,000 on the basis of membrane filtration.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error