Volume 24, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Antigen suspensions of enzyme-treated, formalin-fixed promastigotes of three species of (, and ) were evaluated by using the direct agglutination test with serum samples from healthy individuals and patients with confirmed cases of visceral and cutaneous leishmaniasis. High cross-reactivity was obtained with the three antigens in tests with leishmania sera. Sera from patients with kala-azar were 61% to 96% positive when tested with all three antigens. In tests with sera from patients with American cutaneous leishmaniasis, 81% were positive with antigen, whereas only 54% to 55% were positive with the other two antigens. Normal sera were reactive at low dilutions of 1:16 or less with and antigens. Normal sera tested with antigen reacted to a titer of 128.


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