Volume 23, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The possibility was explored of employing the indirect hemagglutination (IHA) test for the detection and identification of Colorado tick fever (CTF) virus as well as for the detection of antibodies to this virus. For this purpose, sheep erythrocytes fixed with acrolein or glutaraldehyde were sensitized with immune gamma-globulin isolated from ascitic fluid of mice immunized with CTF virus. IHA reaction employing antibody-coated erythrocytes was found to be highly specific and simple to perform, and permitted reading of the results after 1.5 to 2 hours. Preliminary fixation of erythrocytes made it possible to obtain a stable preparation that could be stored without any appreciable loss of activity during an observation period of 3 to 4 months. The IHA test can be applied for the laboratory diagnosis of Colorado tick fever.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...


Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error