Volume 23, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Promastigotes of Panamanian strains of and were inoculated intravenously into 14-day chick embryos. The course of infection was followed by examination of liver impression smears prepared from embryos incubated at 28, 33, and 35°C for 1 hour and 1, 2, and 4 or 6 days post-infection. Parasites of each species declined in numbers rapidly at 35°C. Parasites multiplied more rapidly at 28 than at 33°C, and greater numbers of parasites were observed in the liver after 4 days of incubation at 28°C than after 6 days at 33°C. Multiplication of the two species was similar at 28 and 33°C, respectively. Morphologically, in the liver of chick embryos was more compact and stained more intensely than . Although was generally pale-staining and swollen in appearance, the parasites were viable as demonstrated in cultures initiated with infected liver. Promastigote and amastigote forms were observed in embryos incubated at 28°C, while no flagellum was seen on forms at 33°C.


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