Volume 23, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Parenteral inoculation of mosquitoes was found to be a much more sensitive method to detect dengue viruses than was plaque assay in LLC-MK cells—the most sensitive dengue isolation system known heretofore. This was true whether the viruses were present in sera from naturally infected humans or had been “adapted” to LLC-MK cells or newborn mice. Male mosquitoes were as susceptible to infection as females and could be used without the safety precautions necessary for the latter. All four types of dengue virus replicated to high titer in both male and female mosquitoes and high titered pools of virus could be prepared from these insects without the possible modifying effects of “adaptation” to cell cultures or mice.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error