Volume 21, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The rocker dilution technique was adapted for in vitro testing of antimalarial drugs on five strains of . Seven antimalarial drugs, chloroquine (concentrations: 0.0075, 0.015, 0.03, 0.06, 0.12, 0.3, 0.6, 1.2 µg/ml culture), amodiaquine (0.12 and 0.3 µg/ml), pyrimethamine (3.6 and 5.5 µg/ml), quinine (2.5 and 5.0 µg/ml), cycloguanil (0.144 µg/ml), trimethoprim (50.0 µg/ml) and dapsone (2.5 µg/ml) were tested. The various strains of were classified as susceptible or resistant to a drug depending upon the concentration of agent required to: a) inhibit maturation of rings to trophozoites (i.e., prevent cytoplasmic growth); b) block formation of schizonts and segmenters (i.e., prevent nuclear multiplication); or c) produce aberrant distribution of chromatin within the parasite. A strain of obtained from Arnold and Martin is resistant to pyrimethamine and quinine, but susceptible to chloroquine. The Malayan-Camp. strain is susceptible to chloroquine. The Vietnam-Oak Knoll strain is resistant to chloroquine. Two strains (the Uganda-Palo Alto and the Vietnam-Monterey) were studied in greater detail. Of the five strains studied, the Uganda-Palo Alto strain proved to be the most susceptible and the Vietnam-Monterey strain the most resistant to chloroquine. Both of these strains are susceptible to amodiaquine, cycloguanil and dapsone. The Uganda-Palo Alto strain is resistant to trimethoprim and pyrimethamine, while the Vietnam-Monterey strain is susceptible to these two drugs.


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