Volume 20, Issue 5
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



A dengue vector-control program was conducted on Koh Samui, an island in the Gulf of Thailand, in 1968. Virus-isolation data gathered in support of this program indicated that a single dengue serotype, dengue-4, was active at the time; 63 dengue isolates from mosquitoes and 2 from patients were identified as type 4, the same serotype that prevailed on the island in 1967. Variability was noted in neutralization of many strains by a single lot of type-specific immune serum, as had been observed in previous studies of low-passage tissue-culture strains of dengue virus. Dengue viruses were isolated from both and , the former having the higher infection rate. All dengue isolates were made from in or around homes of patients with laboratory evidence of dengue infection. The sensitivity of the LLC-MK delayed-plaque system was emphasized in these studies since only 66.7% of isolates were recovered with the standard plaque assay.


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