Volume 19, Issue 6_Part_2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Arbovirus Infections Unit, National Communicable Disease Center, Atlanta, Georgia, U.S.A., 11 April 1969.


Isolated by John Davie at the National Communicable Disease Center from heart tissue of adult male captured 5 January 1965 in box trap at Cowbone, Hendry County, Florida, Lat. 26° 17′ N, Long. 81° 5′ W. Open sandy grassland with limestone outcrops. Elevation less than 25 feet. Vegetation: slash pine, palmetto, shrubs, and grasses. Tissue stored at -60°C until tested.

Heart tissue suspension prepared in 20% inactivated rabbit serum in saline solution containing penicillin and streptomycin. Inoculated 0.02 ml ic in 2- to 4-day-old mice on 3 March 1965.

Virus reisolated. No other viruses of this group ever in the laboratories; 26 additional strains isolated by the Epidemiology Research Center.

Labile at 56°C for 30 minutes. RNA virus.


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