Volume 19, Issue 6_Part_2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Gustavo Justines, Middle America Research Unit, Box 2011, Balboa Heights, Canal Zone, 21 August 1969.


Isolated by Gustavo Justines at Middle America Research Unit from pool of 18 adult female, unfed collected 20 December 1962 in light trap at Gamboa, Canal Zone, Republic of Panamá, Lat. 9° 08′ N, Long. 79° 42′ W. Semi-deciduous forest at river margin. Light trap 5 feet above ground. Mosquitoes stored at 4°C until inoculation in mice.

Whole mosquitoes triturated in 25% normal rabbit serum in phosphate-buffered saline solution, pH 7.2. Inoculated 0.02 ml ic in 2-day-old mice on 21 December 1962.

Virus reisolated.

Filterable through Millipore 220 nm without loss of titer, but not the 100 nm. Size estimated by filtration at 70 to 150 nm. RNA virus. Sensitive to 1:100 sodium deoxycholate; control titer 10, after-treatment titer <10.


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