Volume 19, Issue 6_Part_2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


R. H. Kokernot, University of Texas, School of Public Health, P.O. Box 20186, Houston, Texas 77025, 14 August 1969.


Isolated by R. H. Kokernot and colleagues at the University of Illinois, Urbana, Illinois, from a single nymphal (Linn.) removed from a woodchuck () captured 20 June 1967 in the Land Between the Lakes area, western Kentucky, Lat. 36° 50′ N, Long. 88° 00′ W. Oak-hickory forest, border of abandoned fields and deciduous forest. Tick stored over solid CO until tested.

Nymph ground in 2.5 ml of 20% normal rabbit serum in saline solution containing penicillin and streptomycin. Inoculated 0.02 ml in 1- to 2-day-old mice on 8 September 1967.

Virus reisolated from original tick suspension.

Filterable through Seitz asbestos pad and Millipore 220 nm; drop in titer with Millipore 100 nm; not filterable through 50 nm APD.


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