Volume 19, Issue 6_Part_2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645


Gustavo Justines, Middle America Research Unit, Box 2011, Balboa Heights, Canal Zone, 12 June 1969.


Isolated by Gustavo Justines, at Providencia, San Joaquín area, Bolivia, from 15 adult collected 3 September 1963 by hand from walls and floors of a house in Providencia, Lat. 13° S, Long. 64° 30′ W. Flat plain, savanna, and dry forest. Kept alive until processed.

Tick tissues ground in 25% normal rabbit serum in phosphate-buffered saline solution, pH 7.2. Inoculated 0.02 ml ic in 1-day-old mice on 4 September 1963.

Virus reisolated. Another isolate was made from ticks collected at the same time.

Filterable through Millipore 100 nm but not 50 nm; size estimated at 30 to 80 nm. RNA virus. Sensitive to 1:100 sodium deoxycholate; control titer 10, after-treatment titer <10.


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