Abstract

Information from: Fatima Begum and C. L. Wisseman, Jr., Department of Microbiology, University of Maryland, 660 W. Redwood Street, Baltimore, Maryland 21201, U.S.A., 25 July 1967.

Antigenic group: Congo.

Original source: Isolated by Fatima Begum at Lahore, West Pakistan, from Ixodes redikorzevi collected from Alticola trapped 20 July 1964 at Gitidas, Hazara District, West Pakistan, Lat. 35° 07′ N, Long. 73° 59′ E. Habitat: Alpine meadows, subarctic terrain, Kaghan Valley, elevation 12,500 feet. Storage: Specimen stored until processed in -195.8°C liquid nitrogen cylinder.

Method of isolation in laboratory: Ground suspension diluted in 0.75% bovine albumin in borate-saline solution, pH 9. Inoculated 0.01 ml ic in suckling Swiss albino mice on 27 July 1964.

Validity of isolation: Virus reisolated.

Properties of virus: Physical: Filterable through Swinny type Millipore 220 nm. Chemical: No data. Resistance to chemicals: Sensitive to ether (concentration not given); control titer 107.2, after-treatment titer 102.5. Sensitive to 1:100 sodium deoxycholate; control titer 104.5, after-treatment titer 101.5.