Volume 18, Issue 4
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Infectiousness was compared for local, low passage and for prototype strains of dengue-1 through 4 and chikungunya viruses in suspensions of normal females and in mosquito-free controls. Mosquito suspensions reduced the formation of virus plaques by 10- to 2,300-fold in LLC-MK cells. A similar reduction of Japanese encephalitis virus plaques was observed in the presence of suspensions of and . This reduction was not as apparent in suckling mice, however. Thus, with certain virus-host systems, mosquito suspensions per se may prevent the detection of small amounts of arboviruses in pools of these insects when virus is detected directly by plaque formation in cell cultures.


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