Volume 18, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



This study was performed to evaluate a washed-cell, thick-smear antigen for use in the indirect fluorescent-antibody test for malaria. Thick-smear antigens of , and were prepared from parasitized erythrocytes washed free of soluble serum components by several changes of phosphate-buffered saline solution, pH 7.2. Serum samples both positive and negative for malaria antibody were tested with blind and double-blind techniques. The lowest significant diagnostic titer was found to be 1:16. At this dilution, false positive rates were less than 0.5%, false negative rates were below 5%, and the range of titer reproducibility was plus or minus one fourfold dilution. Use of the modified antigen had certain advantages over the conventional whole-blood, thin-smear antigen. Binding of the antigen donor's antibody to his plasmodia is avoided. This effect, previously attributed to an reaction, seems to occur . Reading is facilitated by the large number of plasmodia per field.


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