Volume 17, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Immunoglobulins (IgG, IgA, and IgM) were separated from the serum of a person infected with (Serum T) and another infected with (Serum E) by Sephadex G-200 and DEAE cellulose column chromatography and sucrose-density gradient ultracentrifugation. The composition of fractions was assayed by the Ouchterlony technique and by immunoelectrophoresis. Whole serum and serum fractions were tested by serodiagnostic methods and by passive cutaneous anaphylaxis (PCA) in the guinea pig and monkey. The IgG fractions of Serum E were reactive in serologic tests and in the skin of guinea pigs. Reactivity in the skin of monkeys appeared to be associated with the IgA fraction from a Sephadex G-200 column and the IgG fraction from a DEAE cellulose column. The IgM fraction from serum T showed the greatest reactivity in serologic tests. The IgG fraction was reactive in serologic tests and in the skin of guinea pigs. Neither serum T nor fractions derived from it reacted in the PCA test carried out in monkeys.


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