1921
Volume 16, Issue 6
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
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Abstract

Summary

A semisynthetic medium that promotes growth of under axenic conditions is described. This medium, when enriched with vitamin E, promotes reproduction of the mollusk. The snail was reared over three consecutive generations under germ-free conditions. The sterilization temperature of the medium is critical: when sterilized at higher temperatures than 110°C, the medium does not promote growth.

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/content/journals/10.4269/ajtmh.1967.16.792
1967-11-01
2017-09-22
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