Volume 16, Issue 1
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Rhesus monkeys () can be used for production of monotypic antisera against California encephalitis, Sicilian sand-fly fever, and Turlock arboviruses. Antisera produced with infected smb inoculated intramuscularly were specific in HI tests, and relatively nonspecific in CF tests. In the latter tests, the antisera cross-reacted principally because of nonspecific antibodies directed against normal mouse CNS antigens. Such cross-reactions were not encountered in sera obtained by hyperimmunization with infected cell culture-adjuvant mixtures.

Intracerebral inoculation of these viruses in monkeys resulted in fever, and except in one animal, no other signs pathognomonic of CNS disease. Four of six monkeys had low levels of antibodies; all had antibodies following intramuscular boosters. The antibody levels were below those obtained by hyperimmunization with virus-adjuvant mixtures. Pathologic lesions in the CNS of monkeys strongly suggest that California virus produced mild encephalomyelitis. California encephalitis and Turlock viruses were propagated in cell cultures. Sicilian sand-fly virus did not regularly lyse cells of those cultures tried. These three arboviruses were inactivated by treatment with 0.05% beta-propiolactone.


Article metrics loading...

The graphs shown below represent data from March 2017
Loading full text...

Full text loading...

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error