Volume 16, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Various procedures were tried for improving the quality of group C arbovirus hemagglutinins derived from suckling-mouse liver. After separation of hemagglutinating (HA) and complement-fixing antigens by centrifugation, liver preparations were still too insensitive for use in hemagglutination-inhibition (HI) tests. Sonication produced marked improvement in HA titer, with no evidence that this was due to splitting of the virus particle; however, HI reactivity was still inadequate. Subsequent adsorption onto calcium phosphate (brushite form) followed by stepwise elution resulted in antigens of high quality.

Modified methods are described for preparation of sucrose-acetone antigens and for acetone extraction of immune mouse ascitic fluids.


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