Volume 16, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



The antigenicity of five strains of capable of multiplying at 25°C or lower, as well as at 35°C, was compared with that of eight strains of classic . All strains were grown at 35°C and were exposed to the same dilution of a single fluorescent anti- serum mixed with nonfluorescent normal or anti- sera. Fluorescence was measured with a microfluorimeter. Both groups showed a spectrum of fluorescent brightnesses for their component strains, but the average was 36.2 for body-temperature strains and only 21.2 for the reduced-temperature group. Clear distinction, without overlap between the two groups, occurred in inhibition reactions with a single unlabeled antiserum.

Three lines from a single strain of body-temperature amebae, cultured separately for several years under different conditions and tested after 1½ to 9 months under the same cultural conditions, showed significant differences in brightness. However, the inhibition pattern for all three strains was comparable to that of the other body-temperature amebae.

Reactions of a single strain of were indistinguishable from those of reduced-temperature . On the other hand, a strain of was clearly different from any of the strains on the basis of its inhibition pattern.

None of the reduced-temperature and neither nor were infective to guinea pigs. Six out of nine cultures of body-temperature infected guinea pigs, causing mild to extensive pathologic changes in the cecum. The other three strains were noninfective.


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