Volume 14, Issue 2
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645



Two lots of rabbit antiglobulins, produced against the yeast form of this fungus, were conjugated with fluorescein isothiocyanate. Both reagents brightly stained elements of the yeast and mycelial forms of 15 isolates of . In addition they cross reacted with the tissue form of and other heterologous fungi. Adsorption of one lot of labeled antibodies two times with yeast cells of and once with cells of the mycelial form of rendered this conjugate specific for the yeast form of . Adsorption of the second conjugate once with mycelial growth of followed by a single adsorption with yeast cells of and one adsorption with cells of rendered it specific for the yeast form of .

The specific reagents stained cells in smears of clinical materials from four spontaneous human cases of South American blastomycosis.

The specific reagents did not react with elements of the mycelial form of .


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