1921
Volume 12, Issue 3
  • ISSN: 0002-9637
  • E-ISSN: 1476-1645
USD

Abstract

Summary

Hydatid scolices were incubated axenically in a non-nutrient salt solution (Krebs-Ringer's) at different temperatures, oxygen tensions and osmotic concentrations. Their survival time, rate of evagination and rate of vesicularization were noted. Survival time varied inversely as temperature (5–37°C) and was favored by aerobic and isosmotic conditions. Rates of evagination and vesicularization were not appreciably altered by changes in the tonicity of the medium (even at solution concentrations of from .25× to 2× isosmotic). Absence of single salts from Krebs-Ringer's solution, in particular MgSO and KHPO, decreased survival time. Maximum survival time in Krebs-Ringer's solution was 56 days at 5–9°C, and 44 days at 30°C. In diluted hydatid cyst fluid and diluted sheep serum, scolices survived at 30°C for 64 and 68 days, respectively.

The respiratory rate of scolices was higher too in serum and in hydatid cyst fluid than in either Krebs-Ringer's solution or phosphate buffer. Gentian violet (0.001M), tartar emetic (0.01M and emetine HCl (0.01M) markedly inhibited scolex respiration . Acriflavine (0.01M) inhibited respiration to a lesser degree. Physostigmine first depressed then stimulated respiration. Stibophen, diethylcarbamazine, tetracycline, oxytetracycline, and atropine sulphate had no effect.

Loading

Article metrics loading...

/content/journals/10.4269/ajtmh.1963.12.338
1963-05-01
2017-11-22
Loading full text...

Full text loading...

http://instance.metastore.ingenta.com/content/journals/10.4269/ajtmh.1963.12.338
Loading

Most Cited This Month

This is a required field
Please enter a valid email address
Approval was a Success
Invalid data
An Error Occurred
Approval was partially successful, following selected items could not be processed due to error